 UICDIChicago, IL · Flames | Missouri Valley | David Nikolic6th season since 2021 | 26 | |
 UMassDIAmherst, MA · Minutewomen | MAC | Jason Dowiak | 29 | |
 UMass LowellDILowell, MA · River Hawks | America East | Mira Novak | 24 | |
 UMBCDICatonsville, MD · Retrievers | America East | Rick Stainton4th season since 2023 | 29 | |
 UNC AshevilleDIAsheville, NC · Bulldogs | Big South | Andy McNab | 29 | |
 UNC GreensboroDIGreensboro, NC · Spartans | Southern | Stefani Workman3rd season since 2024 | 27 | |
 UNC WilmingtonDIWilmington, NC · Seahawks | CAA | Chris Neal | 32 | |
 Union CollegeDIIISchenectady, NY | Liberty League (LL) | Karen Perron | 29 | |
 Union UniversityDIIJackson, TN · Bulldogs | Gulf South Conference | Josh Alford | 27 | |
 United States Coast Guard AcademyDIIINew London, CT | NEWMAC | Not listed | 23 | |
 United States Merchant Marine AcademyDIIIKings Point, NY | Skyline Conference (SC) | Not listed | 19 | |
 University of Alabama in HuntsvilleDIIHuntsville, AL · Chargers | Gulf South Conference | James Ballamy | 30 | |
 University of BridgeportDIIBridgeport, CT · Purple Knights | Central Atlantic Collegiate Conference | Tania Armellino | 35 | |
 University of California-Santa CruzDIIISanta Cruz, CA | Coast-to-Coast Athletic Conference (CAC) | Lauren Nadler | 32 | |
 University of California, Merced (UC Merced)DIIMerced, CA · Golden Bobcats | California Collegiate Athletic Association | Roman DuChateau | 42 | |
 University of Central MissouriDIIWarrensburg, MO · Jennies | Mid-America Intercollegiate Athletics Association | Lewis Theobald20th season since 2007 | 25 | |
 University of Central OklahomaDIIOklahoma, OK · Bronchos | Mid-America Intercollegiate Athletics Association | Amel Muhamedagic | 32 | |
 University of CharlestonDIICharleston, WV · Golden Eagles | Mountain East Conference | Sarah Leiby-Gill | 27 | |
 University of ChicagoDIIIChicago, IL | University Athletic Association (UAA) | Amy Howley Reifert36th season since 1991 | 33 | |
 University of Colorado Colorado SpringsDIIColorado Springs, CO · Mountain Lions | Rocky Mountain Athletic Conference | J. B. Belzer | 27 | |
 University of DallasDIIIIrving, TX | Southern Collegiate Athletic Conference (SCAC) | Jeremiah Narvaez | 19 | |
 University of DubuqueDIIIDubuque, IA | American Rivers Conference (ARC) | Not listed | 36 | |
 University of FindlayDIIFindlay, OH · Oilers | Great Midwest Athletic Conference | Sierra Tom | 32 | |
 University of HartfordDIIIWest Hartford, CT | Conference of New England (CNE) | John Natale | 27 | |
 University of Hawai'i at HiloDIIHilo, HI · Vulcans | Pacific West Conference | Gene Okamura | 33 | |
 University of Illinois at SpringfieldDIISpringfield, IL · Prairie Stars | Great Lakes Valley Conference | Scott Myers | 32 | |
 University of IndianapolisDIIIndianapolis, IN · Greyhounds | Great Lakes Valley Conference | Nick Smith | 30 | |
 University of JamestownDIIJamestown, ND · Jimmies | Northern Sun Intercollegiate Conference | Nick Becker | 30 | |
 University of La VerneDIIILa Verne, CA | Southern California Intercollegiate Athletic Conference (SCIAC) | Jeffrey Joyner | 27 | |
 University of LynchburgDIIILynchburg, VA | Old Dominion Athletic Conference (ODAC) | Dr. Todd Olsen33rd season since 1994 | 34 | |
 University of Maine at FarmingtonDIIIFarmington, ME | North Atlantic Conference (NAC) | Not listed | 23 | |
 University of Maine at Presque IsleDIIIPresque Isle, ME | North Atlantic Conference (NAC) | John Ala | 20 | |
 University of MaryDIIBismarck, ND · Marauders | Northern Sun Intercollegiate Conference | Sarah Cook | 28 | |
 University of Mary Hardin-BaylorDIIIBelton, TX | American Southwest Conference (ASC) | Barry Elkins15th season since 2012 | 31 | |
 University of Mary WashingtonDIIIFredericksburg, VA | Coast-to-Coast Athletic Conference (CAC) | Scott Gerseny | 34 | |
 University of Massachusetts-BostonDIIIBoston, MA | Little East Conference (LEC) | Amy Zombeck | 26 | |
 University of Massachusetts-DartmouthDIIINorth Dartmouth, MA | Little East Conference (LEC) | Bobby Desilets | 22 | |
 University of Minnesota CrookstonDIICrookston, MN · Golden Eagles | Northern Sun Intercollegiate Conference | Danny Burstein | 24 | |
 University of Minnesota DuluthDIIDuluth, MN · Bulldogs | Northern Sun Intercollegiate Conference | Greg Cane33rd season since 1994 | 32 | |
 University of Minnesota-MorrisDIIIMorris, MN | Upper Midwest Conference (MWC) | Colin Kaurich | 24 | |