 Union UniversityDIIJackson, TN · Bulldogs | Gulf South Conference | Steven Cox9th season since 2018 | 29 | |
 United States Coast Guard AcademyDIIINew London, CT | NEWMAC | Chris Parsons25th season since 2002 | 31 | |
 United States Merchant Marine AcademyDIIIKings Point, NY | Skyline Conference (SC) | Drew McCarthy | 31 | |
 University of Alabama in HuntsvilleDIIHuntsville, AL · Chargers | Gulf South Conference | John Carrier9th season since 2018 | 30 | |
 University of BridgeportDIIBridgeport, CT · Purple Knights | Central Atlantic Collegiate Conference | Peter Doneit | 33 | |
 University of California-Santa CruzDIIISanta Cruz, CA | Coast-to-Coast Athletic Conference (CAC) | Michael Runeare15th season since 2012 | 26 | |
 University of California, Merced (UC Merced)DIIMerced, CA · Golden Bobcats | California Collegiate Athletic Association | Albert Martins | 41 | |
 University of CharlestonDIICharleston, WV · Golden Eagles | Mountain East Conference | Daniel Smee7th season since 2020 | 53 | |
 University of ChicagoDIIIChicago, IL | University Athletic Association (UAA) | Phillip Kroft4th season since 2023 | 27 | |
 University of Colorado Colorado SpringsDIIColorado Springs, CO · Mountain Lions | Rocky Mountain Athletic Conference | Lewis Wilcox6th season since 2021 | 32 | |
 University of DallasDIIIIrving, TX | Southern Collegiate Athletic Conference (SCAC) | Chase Reynolds | 28 | |
 University of DubuqueDIIIDubuque, IA | American Rivers Conference (ARC) | Andrew Mathys3rd season since 2024 | 38 | |
 University of FindlayDIIFindlay, OH · Oilers | Great Midwest Athletic Conference | Andy Smyth38th season since 1989 | 45 | |
 University of HartfordDIIIWest Hartford, CT | Conference of New England (CNE) | Mike Kulas3rd season since 2024 | 29 | |
 University of Hawai'i at HiloDIIHilo, HI · Vulcans | Pacific West Conference | Diego Marroquin3rd season since 2024 | 32 | |
 University of Illinois at SpringfieldDIISpringfield, IL · Prairie Stars | Great Lakes Valley Conference | Graham Shaw3rd season since 2024 | 30 | |
 University of IndianapolisDIIIndianapolis, IN · Greyhounds | Great Lakes Valley Conference | Gabe Hall5th season since 2022 | 63 | |
 University of JamestownDIIJamestown, ND · Jimmies | Independent | Justin Gilfert1st season since 2026 | 40 | |
 University of La VerneDIIILa Verne, CA | Southern California Intercollegiate Athletic Conference (SCIAC) | Drew Rea | 28 | |
 University of LynchburgDIIILynchburg, VA | Old Dominion Athletic Conference (ODAC) | Chris Yeager | 34 | |
 University of Maine at FarmingtonDIIIFarmington, ME | North Atlantic Conference (NAC) | Blake Hart | 27 | |
 University of Maine at Presque IsleDIIIPresque Isle, ME | North Atlantic Conference (NAC) | John Gonzalez | 20 | |
 University of Mary Hardin-BaylorDIIIBelton, TX | American Southwest Conference (ASC) | Ben Allen5th season since 2022 | 49 | |
 University of Mary WashingtonDIIIFredericksburg, VA | Coast-to-Coast Athletic Conference (CAC) | Jason Kilby | 32 | |
 University of Massachusetts-BostonDIIIBoston, MA | Little East Conference (LEC) | Jake Beverlin | 27 | |
 University of Massachusetts-DartmouthDIIINorth Dartmouth, MA | Little East Conference (LEC) | Andrew Schaefer | 34 | |
 University of Minnesota-MorrisDIIIMorris, MN | Upper Midwest Conference (MWC) | Blake Ordell3rd season since 2024 | 48 | |
 University of Missouri-St. LouisDIISt. Louis, MO · Tritons | Great Lakes Valley Conference | Josh Alderson3rd season since 2024 | 27 | |
 University of MontevalloDIIMontevallo, AL · Falcons | Gulf South Conference | Bruce Dietterle13th season since 2014 | 34 | |
 University of Mount OliveDIIMount Olive, NC · Trojans | Conference Carolinas | Ian Spooner | 41 | |
 University of Mount Saint VincentDIIIBronx, NY | Skyline Conference (SC) | Max Watson | 35 | |
 University of Mount UnionDIIIAlliance, OH | Ohio Athletic Conference (OAC) | David Krems7th season since 2020 | 44 | |
 University of New EnglandDIIIBiddeford, ME | Conference of New England (CNE) | Peter Ostergaard | 36 | |
 University of North GeorgiaDIIDahlonega, GA · Nighthawks | Peach Belt Conference | Anel Lilic4th season since 2023 | 31 | |
 University of Northwestern-St PaulDIIISaint Paul, MN | Upper Midwest Conference (MWC) | Greg Wheaton27th season since 2000 | 40 | |
 University of Pittsburgh at JohnstownDIIJohnstown, PA · Mountain Cats | Pennsylvania State Athletic Conference | Eric Kinsey | 35 | |
 University of Pittsburgh-BradfordDIIIBradford, PA | Allegheny Mountain Collegiate Conference (AMCC) | Not listed | 32 | |
 University of Pittsburgh-GreensburgDIIIGreensburg, PA | Allegheny Mountain Collegiate Conference (AMCC) | Marc Bucci7th season since 2020 | 37 | |
| University of Puerto Rico at Bayamón DIIBayamón, PR · Cowboys | Independent | Not listed | — | No roster |
 University of Puget SoundDIIITacoma, WA | Northwest Conference (NWC) | Steve Mohn3rd season since 2024 | 30 | |