 The University of the SouthDIIISewanee, TN · Tigers | Southern Athletic Association | NIck DiBernardo7th season since 2021 | 51 | |
 Thiel CollegeDIIIGreenville, PA · Tomcats | Presidents' Athletic Conference | Alex Skvarch4th season since 2024 | 16 | |
 Thomas CollegeDIIIWaterville, ME · Terriers | Massachusetts State Collegiate Athletic Conference | Bill Scott6th season since 2022 | 28 | |
 Thomas More UniversityDIICrestview Hills, KY · Saints | Great Midwest Athletic Conference | Sean McGinnis5th season since 2023 | 29 | |
 Tiffin UniversityDIITiffin, OH · Dragons | Great Midwest Athletic Conference | Travis Brown5th season since 2023 | 52 | |
 TowsonDITowson, MD · Tigers | CAA | Shawn Nadelen5th season since 2023 | 50 | |
 Transylvania UniversityDIIILexington, KY · Pioneers | Heartland Collegiate Men's Lacrosse Conference | Logan Otto6th season since 2022 | 37 | |
 Trine UniversityDIIIAngola, IN · Thunder | Michigan Intercollegiate Athletic Association | Ryan Kuhn6th season since 2022 | 44 | |
 Trinity CollegeDIIIHartford, CT · Bantams | New England Small College Athletic Conference | Taylor Mendoza2nd season since 2026 | 64 | |
 Tufts UniversityDIIIMedford, MA · Jumbos | New England Small College Athletic Conference | Casey D'Annolfo5th season since 2023 | 53 | |
 Tusculum UniversityDIIGreeneville, TN · Pioneers | South Atlantic Conference | Mike Brisbin4th season since 2024 | 42 | |
 UMassDIAmherst, MA · Minutemen | Atlantic 10 | Kyle Smith7th season since 2021 | 46 | |
 UMass LowellDILowell, MA · River Hawks | America East | Not listed | 51 | |
 UMBCDIBaltimore, MD · Retrievers | America East | James Purpura1st season since 2027 | 41 | |
 Union CollegeDIIISchenectady, NY · Garnet Chargers | Liberty League | Derek Witheford | 53 | |
 United States Coast Guard AcademyDIIINew London, CT · Bears | New England Women's and Men's Athletic Conference | Ray LaForte13th season since 2015 | 51 | |
 United States Merchant Marine AcademyDIIIKings Point, NY · Mariners | Skyline Conference | Dan Paccione5th season since 2023 | 40 | |
 University of Alabama in HuntsvilleDIIHuntsville, AL · Chargers | Peach Belt Conference | Jordan Warren3rd season since 2025 | 67 | |
 University of BridgeportDIIBridgeport, CT · Purple Knights | Central Atlantic Collegiate Conference | Ryan McAleavey5th season since 2023 | 15 | |
 University of DubuqueDIIIDubuque, IA · Spartans | Northern Athletics Collegiate Conference | Tyler Thomas4th season since 2024 | 31 | |
 University of HartfordDIIIWest Hartford, CT · Hawks | Conference of New England | Ryan Martin5th season since 2023 | 47 | |
 University of IndianapolisDIIIndianapolis, IN · Greyhounds | Great Lakes Valley Conference | Greg Stocks5th season since 2023 | 54 | |
 University of LynchburgDIIILynchburg, VA · Hornets | Old Dominion Athletic Conf. | Steve Koudelka5th season since 2023 | 58 | |
 University of Mary WashingtonDIIIFredericksburg, VA · 1301 College Ave. | Coastal Lacrosse Conference | Kyle Hofstaedter2nd season since 2026 | 66 | |
 University of Massachusetts-BostonDIIIBoston, MA · UMass Boston | Little East Conference | Tyler Low5th season since 2023 | 42 | |
 University of Massachusetts-DartmouthDIIIDartmouth, MA · Corsairs | Little East Conference | Dan Sharbaugh | — | No roster |
 University of MontevalloDIIMontevallo, AL · Falcons | Peach Belt Conference | Jason Lange10th season since 2018 | 65 | |
 University of Mount OliveDIIMount Olive, NC · Trojans | Conference Carolinas | Dylan Price13th season since 2015 | 46 | |
 University of Mount Saint VincentDIIIBronx, NY · Dolphins | Skyline Conference | James Fitzgerald6th season since 2022 | 26 | |
 University of Mount UnionDIIIAlliance, OH · Purple Raiders | Ohio Athletic Conference | Dominic Perella6th season since 2022 | 57 | |
 University of New EnglandDIIIBiddeford, ME · Nor'easters | Conference of New England | Charles Burch | 31 | |
 University of Pittsburgh-BradfordDIIIBradford, PA · Panthers | Heartland Collegiate Men's Lacrosse Conference | Scotty Gwyn | 28 | |
 University of Saint JosephDIIIWest Hartford, CT · Joey the Blue Jay | Great Northeast Athletic Conference | Andrew Fink4th season since 2024 | 34 | |
 University of ScrantonDIIIScranton, PA · Royals/Lady Royals | Landmark Conference | Michael Hofmeister5th season since 2023 | 53 | |
 University of Southern MaineDIIIGorham, ME · Huskies | Little East Conference | Sam Manders5th season since 2023 | 25 | |
 University of TampaDIITampa, FL · Spartans | Sunshine State Conference | J.B. Clarke4th season since 2024 | 63 | |
| University of the District of Columbia DIIWashington, DC · Firebirds | East Coast Conference | Matthew Marrett | 20 | |
 Ursinus CollegeDIIICollegeville, PA · Bears | Centennial Conference | Ryan Burke | 44 | |
 UtahDISalt Lake City, UT · Utes | ASUN | Andrew McMinn6th season since 2022 | 48 | |
 Utica UniversityDIIIUtica, NY · Pioneers | Empire 8 | Sean Behan4th season since 2024 | 31 | |